CD14 expression and IL-10 production of cells differentiated in presence of IFN-α plus LPS.

The dot plots in panel A were obtained by FACS analysis on day 6 from cultures containing 1000 U/mL IFN-α and/or 1 ng/mL LPS as indicated. Despite homogeneous expression of the DC maturation markers CD83, CD80, and CD86 (not displayed), the expression of CD14 is retained in a subpopulation of cells cultured in the presence of IFN-α plus LPS. Shown are the data from one representative experiment; similar results were obtained in 3 further experiments. Panel B displays the amount of IL-10 found in the supernatants on day 4 (mean ± SD of 3 independent experiments).