American Society of Hematology

Fig. 1.

$Fig. 1. DEAE column chromatography. / The sample (3.6 mg) from the second heparin column was applied to the DEAE column connected to Waters Protein Purification System. (A) Protein elution pattern. The fractions in which the 150-kd band was detected by SDS-PAGE analysis are indicated by a bar. (B) SDS-PAGE analysis. Samples (20 μL) were applied to 6% gel without reduction, and protein bands were stained by Blue stain reagent. Fraction numbers are shown at the bottom. (C) Immunoblotting analysis for the protease activity. After the reactions, samples were run on 7.5% gel with reduction and transferred to membrane. The bands were visualized by alkaline phosphatase substrate. Lane c is a control without the enzyme.$

DEAE column chromatography.

The sample (3.6 mg) from the second heparin column was applied to the DEAE column connected to Waters Protein Purification System. (A) Protein elution pattern. The fractions in which the 150-kd band was detected by SDS-PAGE analysis are indicated by a bar. (B) SDS-PAGE analysis. Samples (20 μL) were applied to 6% gel without reduction, and protein bands were stained by Blue stain reagent. Fraction numbers are shown at the bottom. (C) Immunoblotting analysis for the protease activity. After the reactions, samples were run on 7.5% gel with reduction and transferred to membrane. The bands were visualized by alkaline phosphatase substrate. Lane c is a control without the enzyme.

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