American Society of Hematology

Fig. 2.

$Fig. 2. Gel filtration on Superose-6 column. / A portion of DEAE column fraction was applied to the Superose–6 column. (A) Protein elution pattern. The fractions in which the 150-kd band was detected by SDS-PAGE analysis are indicated by the bar, and the elution positions of fibrinogen (330 kd) and IgG (150 kd) are indicated by arrows. (B) SDS-PAGE analysis. The samples (20 μL) were applied to 6% gel without reduction, and protein bands were visualized by silver staining. (C) Immunoblot analysis for the protease activity. After the reaction, samples were run on 6% gel with reduction and transferred to membrane. The bands were detected by the enhanced chemiluminescent reagent. The fraction numbers are shown in the bottom, and lane c is a control without the enzyme.$

Gel filtration on Superose-6 column.

A portion of DEAE column fraction was applied to the Superose–6 column. (A) Protein elution pattern. The fractions in which the 150-kd band was detected by SDS-PAGE analysis are indicated by the bar, and the elution positions of fibrinogen (330 kd) and IgG (150 kd) are indicated by arrows. (B) SDS-PAGE analysis. The samples (20 μL) were applied to 6% gel without reduction, and protein bands were visualized by silver staining. (C) Immunoblot analysis for the protease activity. After the reaction, samples were run on 6% gel with reduction and transferred to membrane. The bands were detected by the enhanced chemiluminescent reagent. The fraction numbers are shown in the bottom, and lane c is a control without the enzyme.

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