Fig. 3.
Molecular weight estimation and detection of carbohydrate moiety of the purified vWF-cleaving protease.
The samples (30 μL) from fraction 28 of the Superose-6 column (described in Figure 2) were applied to 6% gel after reduction. Lane 1 was stained for protein by Coomassie Blue stain reagent and lane 2 was stained for carbohydrate by periodic acid–Schiff reagent.