Antisense inhibition or blocking cell surface tTG reduces migration of TPA-stimulated THP-1 cells on Fn and its 42-kd fragment.

The 5 × 10⁴ THP-vector (░) and THP-antisense (▨) cells in serum-free AIM-V medium containing 0.5% BSA were stimulated with 150 ng/mL TPA and placed for 4 hours at 37°C into upper chambers of Transwells (Costar) where undersurface of the inserts was precoated with collagen I (Col I), laminin (Ln), Fn, the 110-kd or 42-kd fragments of Fn, or BSA. Then 125 ng/mL MCP-1 was added to lower chambers. Some samples of THP-vector cells were preincubated for 1 hour with function-blocking polyclonal anti-tTG antibody that was kept in the medium during the assay (■). Shown are the means of 3 separate experiments performed in duplicate.