Fig. 3.
Cell line comparisons and Northern blot confirmation analyses.
(A) Array-based transcription patterns for bone marrow (Cy3) versus Cy5-labeled bone marrow (NBM), peripheral blood mononuclear cells (WBC), K562 erythroleukemia cells from 2 sources (K562A, K562B), and the HEL erythroleukemia cell line. K562B RNA was the source used for all subsequent E-chip hybridizations in this study. (B) Northern blots probed for representative proliferation- and differentiation-associated genes. Each lane contained 2.5 μg mRNA from carbonic anhydrase I (CAI), CD36, Stathmin (STATH), DEKoncogene (DEK), and hembase clone Ad08c04 (Ad08c04).