American Society of Hematology

Fig. 5.

$Fig. 5. PIP3 production in fMLP and ANCA-stimulated neutrophils. / 2 × 107 neutrophils were primed with 2 ng/mL TNF-α and stimulated either with 1 μM fMLP, 250 μg/mL MPO-ANCA, 250 μg/mL PR3-ANCA, or 250 μg/mL normal and PIP3 production measured. (A) Representative HPLC trace showing inositol phosphates derived from 30-second fMLP-stimulated 32P-labeled neutrophils (●) superimposed on trace 3H internal standards (■). (B) Graph showing percentage dpm in 32P PIP3 fraction after 30-second and 15-minute stimulation. All samples were tested in duplicate, and results show pooled data from 2 experiments. ■ indicates cells; ░, fMLP; ▥, MPO-ANCA; ▤, PR3-ANCA; ▨, normal IgG.$

PIP₃ production in fMLP and ANCA-stimulated neutrophils.

2 × 10⁷ neutrophils were primed with 2 ng/mL TNF-α and stimulated either with 1 μM fMLP, 250 μg/mL MPO-ANCA, 250 μg/mL PR3-ANCA, or 250 μg/mL normal and PIP₃ production measured. (A) Representative HPLC trace showing inositol phosphates derived from 30-second fMLP-stimulated ³²P-labeled neutrophils (●) superimposed on trace ³H internal standards (■). (B) Graph showing percentage dpm in ³²P PIP₃ fraction after 30-second and 15-minute stimulation. All samples were tested in duplicate, and results show pooled data from 2 experiments. ■ indicates cells; ░, fMLP; ▥, MPO-ANCA; ▤, PR3-ANCA; ▨, normal IgG.

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