HAGBP affects Bcr-Abl–dependent proliferation of 32D and NIH3T3 cells, but not the proliferation of 32D cells in the presence of IL-3 or v-Raf–expressing NIH3T3 cells.

(A) A total of 10⁵ IL-3–dependent 32D mouse myeloid cells grown in the presence of IL-3 or the same number of IL-3–independent Bcr-Abl–expressing 32D cells (32D-p210^Bcr-Abl) grown without IL-3 were incubated with 20 μM inactive control peptide (P7) or Antp-SS–HAGBP (P6) as indicated. Peptide was applied twice, once initially and once after 24 hours. Viable cells were counted after 48 hours. Error bars indicate SEM (n = 6). One hundred percent corresponds to approximately 6.5 × 10⁵ cells obtained in the absence of exogenously added peptide at the end of the incubation period. (B) A total of 10⁵ 3T3 v-Raf cells or 3T3 Bcr-Abl cells were grown in the presence of 5% FBS without peptide addition or with 20 μM of the peptides indicated (P6 = Antp-SS–HAGBP; P7 = control peptide). One hundred percent corresponds to approximately 2 × 10⁵ untreated 3T3 v-Raf cells or approximately 3.5 × 10⁵ 3T3 Bcr-Abl cells at the end of the incubation period.