HAGBP inhibits proliferation of freshly isolated preparations enriched with blast cells from human CML patients.

Blast cells were isolated from 16 patients with active disease by density gradient centrifugation and equilibrated for 16 to 24 hours in culture medium at 37°C before peptide application. Peptides were added initially at a concentration of 20 μM, and the same dose was added again after 24 hours. Viable blast cells were counted after 48 hours. Blast cell number without peptide addition equals 100% proliferation. Error bars indicate SEM (n = 3 to 5). Isolates in which a significant inhibitory effect is seen on proliferation with HAGBP (P6; white bars) versus control peptide (P7; gray bars) are indicated by bold and underlined numbers.