Fig. 1.
Effect of VT-1 on platelet adhesion and thrombus formation on HMEC-1 and HUVECs.
HMEC-1 and HUVECs were treated for 24 hours with VT-1 (0, 2, 10, 50 pM) and then perfused with mepacrine-labeled blood at high shear stress (60 dynes/cm2). The area occupied by thrombi was measured on images acquired by confocal fluorescence microscopy, as described in “Materials and methods.” Data are expressed as mean ± SE of area covered by thrombi (n = 8 experiments). *P < .01 versus untreated cells; °P < .05 HUVEC + VT-1(10 pM) versus HMEC-1 + VT-1 (10 pM).
