Fig. 2.
IL-2–induced proliferation is inhibited by mAbs specific for the GPI-anchored proteins Thy-1, Ly-6A/E, and CD48.
The expression of Thy-1, Ly6A/E, and CD48 on GPI+ (A) and GPI− (B) variants of the 2.10 T-cell clone was analyzed by flow cytometry. The first histogram represents staining with secondary antibodies alone. (C) GPI+ and GPI− 2.10 T cells were cultured in wells precoated with the indicated mAbs and 15 U/mL IL-2. Uptake of 3H-thymidine is represented as the percentage of the proliferative response to IL-2 in the absence of added mAbs.
