American Society of Hematology

Fig. 6.

$Fig. 6. JAK1 and JAK3 are localized in detergent-soluble membranes. / CTLL-2 cells were left untreated or were stimulated for 10 minutes with IL-2 before lysis and sucrose density gradient ultracentrifugation. JAK1 and JAK3 were immunoprecipitated from pooled fractions of sucrose gradients corresponding to lipid rafts (R) and soluble membranes (S). Immunoprecipitations were split in 2 and resolved by SDS-PAGE, and immunoblotting was performed using phosphotyrosine (PY)–, JAK1-, or JAK3-specific antibodies.$

JAK1 and JAK3 are localized in detergent-soluble membranes.

CTLL-2 cells were left untreated or were stimulated for 10 minutes with IL-2 before lysis and sucrose density gradient ultracentrifugation. JAK1 and JAK3 were immunoprecipitated from pooled fractions of sucrose gradients corresponding to lipid rafts (R) and soluble membranes (S). Immunoprecipitations were split in 2 and resolved by SDS-PAGE, and immunoblotting was performed using phosphotyrosine (PY)–, JAK1-, or JAK3-specific antibodies.

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