American Society of Hematology

Fig. 7.

$Fig. 7. The heterotrimeric receptor complex composed of IL-2 bound to IL-2Rα, IL-2Rβ, and IL-2Rγ is detected in detergent-soluble membranes. / (A) CTLL-2 cells were incubated with 125I-labeled IL-2, and bound IL-2 was cross-linked to cell-surface proteins. After lysis, sucrose density gradient ultracentrifugation was performed. Fractionation (Fr) into lipid rafts and soluble membranes was determined by immunoblotting for GM1. (B) Proteins in fractions corresponding to lipid rafts and soluble membranes were resolved by SDS-PAGE, and proteins cross-linked to 125I-labeled–IL-2 were visualized by autoradiography. Bands corresponding to125I–IL-2 cross-linked to IL-2Rα, IL-2Rβ, and IL-2Rγ are indicated.$

The heterotrimeric receptor complex composed of IL-2 bound to IL-2Rα, IL-2Rβ, and IL-2Rγ is detected in detergent-soluble membranes.

(A) CTLL-2 cells were incubated with ¹²⁵I-labeled IL-2, and bound IL-2 was cross-linked to cell-surface proteins. After lysis, sucrose density gradient ultracentrifugation was performed. Fractionation (Fr) into lipid rafts and soluble membranes was determined by immunoblotting for GM1. (B) Proteins in fractions corresponding to lipid rafts and soluble membranes were resolved by SDS-PAGE, and proteins cross-linked to ¹²⁵I-labeled–IL-2 were visualized by autoradiography. Bands corresponding to¹²⁵I–IL-2 cross-linked to IL-2Rα, IL-2Rβ, and IL-2Rγ are indicated.

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