Disruption of lipid raft integrity does not affect IL-2–induced tyrosine phosphorylation of JAK1 and JAK3.

(A-B) CTLL-2 cells were left untreated or were incubated with 10 mM MCD before stimulation with IL-2. JAK1 and JAK3 were immunoprecipitated from postnuclear lysates. Immunoprecipitations were split in 2 and resolved by SDS-PAGE, and immunoblotting was performed using phosphotyrosine (PY)–, JAK1-, or JAK3-specific antibodies. (C-D) 2.10 cells were left untreated or were incubated with 10 mM MCD. (C) Cells were stimulated with IL-2, and JAK1 immunoprecipitated from postnuclear lysates was immunoblotted using anti-PY or anti-JAK1. (D) Cells were stimulated with anti-TCR, and PLCγ1 immunoprecipitated from postnuclear lysates was immunoblotted using anti-PY or anti-PLCγ1.