Establishment of B-lineage cell lines in ST2/IL-7 cell cultures of lymphohematopoietic progenitors obtained from 11-dpc BALB/c mouse embryos.
(A) rIL-7 dose-dependent emergence of cell clones under limiting dilution conditions at 8 days of culture; · · · · · indicates 0 ng/mL, – – –, 0.3 ng/mL, and _____, 3 ng/mL. One representative experiment of 3 is shown for 11-dpc liver (embryo cells) and for BM cells. (B) Kinetics of establishment of long-term growing embryo and LFL/BM cell lines at 3 ng/mL rIL-7. The cell lines were obtained by seeding 105 embryo or LFL/BM cells in 96-well plates at 1000 cells/well. Data are presented as mean ± SD of 3 independent experiments for embryo (circles) and LFL/BM (triangles) cell lines, and represent the number of B-lineage cell lines growing at each time point.
