Ig V_HDJ_H rearrangements from long-term in vitro growing embryo cell lines.

V_H7183-D-J_H and V_HJ558-D-J_H rearrangements were amplified, cloned, and sequenced as described in “Materials and methods.” The V_HDJ_H joint sequences of clones obtained from the progeny of 2 independent embryo-derived precursors are shown. The sequences are aligned under the germline sequences corresponding to the 3′ region of V_H 7183 and J558 families and the 5′ region of germline JH4 (top line). Dashed lines denote identities. N nucleotides are underlined. Parentheses indicate probable D-D joints. Nucleotides in italics may be assigned to germline sequences of V, D, or J segments and are putative sites of homology-directed recombination. RF indicates reading frame.