Bcr-Abl kinase-active BaF3p185 cells adherent to fibronectin exhibit a significant survival advantage following γ-irradiation.

(A) Bcr-Abl⁺ cells with (dashed line) or without (dotted line) STI571+IL-3 and Bcr-Abl⁻ BaF3 cells (solid line) adherent to fibronectin, laminin, or polylysin were γ-irradiated with indicated dosages. After a growing period of 17 hours, nonadherent cells were removed and survival of adherent cells was measured by using MTT survival assay. The absorbance of the samples was analyzed in an ELISA reader at 570 nm. Data shown are representative for 3 independent experiments. Values are means ± SD of triplicates. (B) Viability of Bcr-Abl⁻ BaF3 cells adherent to fibronectin 17 hours after irradiation with 3, 8, 20, or 50 Gy. Data shown are representative for 3 independent examinations. Values are means ± SD of triplicates.