The PI-3K inhibitor LY294002 completely antagonizes the fibronectin-induced antiapoptotic effect in Bcr-Abl⁺ cells.

Bcr-Abl⁺ BaF3p185 cells cultured on fibronectin were treated without (■) or with PD98059 (░) or LY294002 (■). After a growing period of 24 hours, all cells (adherent and nonadherent cells) were irradiated with 0 Gy or 50 Gy and incubated with or without inhibitor for an additional 8 hours. The percentage of apoptotic cells was then determined by using Annexin V-FITC staining. Values reflect the mean ± SEM of 3 independent examinations.