AKT is hyperphosphorylated in BaF3p185 cells adherent to fibronectin.

Bcr-Abl⁻ BaF3 cells with IL-3 and Bcr-Abl⁺BaF3p185 cells with or without STI571/IL-3 were cultured in serum-free RPMI medium for 4 hours in the presence or absence of 5 μM LY294002. Cells were then plated onto fibronectin (FN)-, polylysin (PL)-, or polyHEMA (pHe)-coated dishes with or without inhibitor and incubated for 45 minutes in serum-free RPMI at 37°C. Lysates were prepared from adherent cells and analyzed by Western blotting by using antiphospho-AKT or anti-AKT.