Adhesion of nonsickle and sickle erythrocytes to immobilized Siglec-6, P-selectin, or P-selectin in the presence of EDTA.

The data shown are the nonstatic adherence (in the rotatory adhesion assay) of untreated or sialidase-treated RBCs to immobilized BSA (■), Siglec-Ig chimera (), P-selectin-Ig chimera (▪), or P-selectin-Ig chimera in the presence of 5 mM EDTA (). The 100% adherence is the mean number of untreated nonsickcle (AA) RBCs/field in a well in which BSA was immobilized. SS, sickle erythrocytes. The data are mean percent adherence for 4 nonsickle and 6 sickle RBC samples. Differences in adherence with P < .05 were determined to be significant. Significant adhesion is compared to adhesion to BSA (*) and to Siglec (§), and significant inhibition of adhesion to P-selectin due to EDTA is denoted by.