Effect of sialidase treatment of nonsickle and sickle erythrocytes on their adherence to HUVECs treated with or without thrombin and to immobilized Siglec-6 or P-selectin.

The data shown are the adherence of RBCs that had or had not been treated with sialidase either to HUVECs, which had been treated with thrombin or medium alone, or to immobilized BSA, Ig-Ig chimera, or P-selectin–Ig chimera. The 100% adherence is the mean number of untreated nonsickle (AA) RBCs/field adherent to untreated HUVECs. SS, sickle erythrocytes. (A) The reduction of static erythrocyte adherence to untreated (■) or thrombin-treated HUVECs due to treatment of the RBCs with sialidase (▪). The data are mean percent adherence from 4 replicate experiments. Significant inhibition due to sialidase withP < .05 was denoted by *. (B) The reduction of nonstatic erythrocyte adherence to immobilized Siglec-6 or P-selectin due to treatment of the RBCs with sialidase., Siglec, untreated;, Siglec, sialidase;, P-seclectin, untreated;, P-selectin, sialidase. The data are mean percent adherence for 3 nonsickle and 5 sickle RBC samples. Significant adhesion (P < .05) is compared to adhesion to Siglec-6 (§) and significant inhibition of adhesion to P-selectin due to sialidase is denoted by .