Fig. 2.
![Fig. 2. Analysis of GMTag protein biologic activity. / The GM-CSF/IL-3 proliferation-dependent NFS-60 cells were incubated with decreasing concentrations of nontransfected (○) or pBK-CMV–GMTAg AU− transfected (●) HeLa cell supernatants. The first dilution of the pBK-CMV–GMTag AU−transfected cell supernatant contained 10 ng/mL GM-CSF as determined by ELISA. (■) Cells were incubated with 100 μL supernatant of pBK-CMV–GMTAg AU− transfected HeLa cells in the presence of decreasing concentrations of antimouse GM-CSF antibody. The transfected cell supernatant contained 20 ng/mL GM-CSF as determined by ELISA. Cell proliferation was measured by [3H]-thymidine incorporation (see “Materials and methods”).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/98/5/10.1182_blood.v98.5.1281/5/h81711446002.jpeg?Expires=1735512098&Signature=Lce4tzdMoFuJ4txeNBvK~5L3lXZXvRLzph7OlQVmyvUdBcQI-vzk8tUm8dcKnf-BVLV1qobmoLUgFtzQ32fI~dAJ4NyMlbml~lhPub0VnmjDdR4rUEApN-eKmeAKwSPF3n8u7aICc8FqN3c6SA0g1sWtZVh6CzEQLJFFWVsMg1NefR29oYrGqibT7F~H7w6~2yokc3yaDeFqyNAb4FX16l-sAML2BsyiuVDUwWPDpjg8RExurT2HqTfdA4hg3EhHrvmjMyWBrrtassiuUwmR0oVRmPQomZ-cIDn6xwCkLBrQw4XIClaaxitMRXQSj26wJgdFxvId6bpVSx9FBPoTdw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Analysis of GMTag protein biologic activity.
The GM-CSF/IL-3 proliferation-dependent NFS-60 cells were incubated with decreasing concentrations of nontransfected (○) or pBK-CMV–GMTAg AU− transfected (●) HeLa cell supernatants. The first dilution of the pBK-CMV–GMTag AU−transfected cell supernatant contained 10 ng/mL GM-CSF as determined by ELISA. (■) Cells were incubated with 100 μL supernatant of pBK-CMV–GMTAg AU− transfected HeLa cells in the presence of decreasing concentrations of antimouse GM-CSF antibody. The transfected cell supernatant contained 20 ng/mL GM-CSF as determined by ELISA. Cell proliferation was measured by [3H]-thymidine incorporation (see “Materials and methods”).
