![Fig. 3. Transgene expression. / Detection under dark field illumination (magnification × 6) of CMV-GMTag AU− (A) and AU+ (B) transgene expression in E14 embryo by in situ hybridization using [35S]-labeled GM-CSF antisense riboprobe; (C) nontransgenic embryo. The white dots observed in the CMV-GMTag AU− sample correspond to dispersed transgenic mRNA-containing cells. White lines appearing in the CMV-GMTag AU+ and nontransgenic samples correspond to artifacts due to dust and manipulation. (D-G) Detection under bright field illumination of CMV-GMTagAU− transgene expression (black dots) (D) and expression foci at greater magnification (× 100) in the choroid plexus (E), the olfactory epithelium (F), and heart (G). (H) Staining of the heart by hemalum-eosin to show transgene expression by cells in clusters in blood vessels (arrows); br indicates brain; cp, choroı̈d plexus; he, heart; oe, olfactory epithelium; and ve, ventricule. These expression patterns are representative of the different embryos analyzed.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/98/5/10.1182_blood.v98.5.1281/5/h81711446003.jpeg?Expires=1735512696&Signature=40emayl~X8CAfsjmqJmr5O83wWaRfFayUZOLwnc7RagUtzv0UM3l9JyEu9njYSNnyi5vlTRjlFWIVb3sGwfAHIGnEkmnAYGDlAeD06ufdWtMrw52Yav4njKhYmM9cM6Oa3rZhGmPu4~9tRWNIO48cRD49H-qqvObIKrd9SmE6ZChXjBMFlAZr1ktR-Y6OmFDXoO0AuFqL-riHtLRP3vwKmwNdPvWOKpPyIwzPxui83WIp14MciB9s0vFLxQWzoXnz0GgK9UOwjJgNWGzH0rEC~EO0RqGJMvLKbFR-X-ZN2DG~KumQx24kk3waPgWm2tgVBbNX7Xgij9Wx6g0tpaR2g__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Transgene expression.
Detection under dark field illumination (magnification × 6) of CMV-GMTag AU− (A) and AU+ (B) transgene expression in E14 embryo by in situ hybridization using [35S]-labeled GM-CSF antisense riboprobe; (C) nontransgenic embryo. The white dots observed in the CMV-GMTag AU− sample correspond to dispersed transgenic mRNA-containing cells. White lines appearing in the CMV-GMTag AU+ and nontransgenic samples correspond to artifacts due to dust and manipulation. (D-G) Detection under bright field illumination of CMV-GMTagAU− transgene expression (black dots) (D) and expression foci at greater magnification (× 100) in the choroid plexus (E), the olfactory epithelium (F), and heart (G). (H) Staining of the heart by hemalum-eosin to show transgene expression by cells in clusters in blood vessels (arrows); br indicates brain; cp, choroı̈d plexus; he, heart; oe, olfactory epithelium; and ve, ventricule. These expression patterns are representative of the different embryos analyzed.
