Fig. 4.
![Fig. 4. Reduced RBC survival in murine sickle cell anemia and thalassemia. / At t = 0, murine RBCs were biotinylated by tail vein injection of sulfo-NHS-biotin. At indicated time intervals, streptavidin labeling and flow cytometric analysis measured the number of biotinylated cells. Survival data were fitted to A(t) = A0[1−(t/T)]exp−kt, in which A(t) is the number of biotinylated RBCs at time (t), A0 is the initial number of biotinylated cells at t = 0, and T is the time of senescent death of RBCs. Data are depicted for 6 different sickle (SS) mice, 4 different β-thalassemic (β-thal) mice, and 3 different control (WT) mice.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/98/5/10.1182_blood.v98.5.1577/5/h81711472004.jpeg?Expires=1735513158&Signature=uO7awSJ2ZOQ22nt7KBfy54WGEyL6v7zP6t~1RuvCgc-Fb5i0~FY0BHE6mYLa1OJPtfCW5IkKSaQ79bQzcBq~OPJjPmsdc9XgExGrEwyg-z8QmGHu3xS1pMVDpILbK2Qt6QewZ9koHvh6x0f672sT8Zj-9mWCTe4HmRWDYKQt2nGrAFiBrdVyyGP3BOoTpNsVb3W~tlkCPX~1dTXVpA3pIWZhvVeFt~DD91T3uvmdx~O8NS6qd8-bK1qvODT9TUiN4ylvXIXSZ4tv4iiJOKkPNEjTSYJm6-yphP5AtkaqFmq-h6tgv-F7v~zcvjDqt0ITcU0Gq4~lNxHmYFaKfxCqoQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Reduced RBC survival in murine sickle cell anemia and thalassemia.
At t = 0, murine RBCs were biotinylated by tail vein injection of sulfo-NHS-biotin. At indicated time intervals, streptavidin labeling and flow cytometric analysis measured the number of biotinylated cells. Survival data were fitted to A(t) = A0[1−(t/T)]exp−kt, in which A(t) is the number of biotinylated RBCs at time (t), A0 is the initial number of biotinylated cells at t = 0, and T is the time of senescent death of RBCs. Data are depicted for 6 different sickle (SS) mice, 4 different β-thalassemic (β-thal) mice, and 3 different control (WT) mice.
