The effect of pharmacological blockade of either the PI3K/Akt pathway or the MAP kinase pathway on apoptosis in primary human erythroid progenitors.

Cells on day 5 to 7 of culture were washed and returned to medium either with or without growth factors (Epo 2 U/mL and SCF 20 ng/mL) or, in the case of cells given growth factors, either with (A) LY294002 (25 μM, n = 8) or (B) U0126 (10 μM, n = 5) as shown, or not. The mean percentage ± SEM of apoptotic cells at 8 hours, as determined by annexin V binding, is indicated.