Effect of TNF on in vitro cellular expansion of CD34⁺ and CD34⁺CD38⁻ cells.

Cells were cultured in SF medium in KFT3 in the absence or presence of TNF (20 ng/mL). CD34⁺ CB cells (5 experiments) were cultured for 5 to 6 days, CD34⁺CD38⁻ CB cells (3 experiments) for 8 days, and CD34⁺CD38⁻ BM cells (4 experiments) for 5 days. Data represent the mean values of all experiments for different cell types. Error bars show the SEM.