Effect of TNF on recruitment of single CB CD34⁺CD38⁻ cells into proliferation.

Single CD34⁺CD38⁻ CB cells were deposited with a single cell depositor, coupled to a FACSVantage, into wells in Terasaki plates containing 20 μL SF medium supplemented with KFT3 in the absence or presence of TNF (20 ng/mL). Wells were inspected on the days indicated for recruitment into proliferation (first cell division; A) and clonal size (B). Results are presented as mean values (SD) of 2 independent experiments. 0 indicates no cell division; NA, not applicable.