TNF negatively regulates maintenance of cycling BM CD34⁺CD38⁻ LTC-ICs.

The 25 to 75 sorted CD34⁺CB38⁻ BM cells and their EEs following 5 to 8 days of culture in KFT3 in the absence or presence of TNF were evaluated for 6-week LTC-CFC activity, as described in “Materials and methods.” Data represent the mean values of 2 to 3 replicates for each group in each of 4 independent experiments.