TNF down-regulates expression of CD34 and promotes differentiation of ex vivo expanded CD34⁺ CB cells.

The upper 3 sets of panels show CD34 and lineage (CD3, CD14, CD15, CD19, CD20, CD56, CD66b, glycophorin A) expression on CD34⁺CB cells (purity 99%) cultured in KFT3 in the absence or presence of TNF (20 ng/mL) for 2, 5, and 12 days. The lower panels show staining with anti-CD34 and 2 myeloid-specific markers (CD14 and 66b) following 12 days of incubation. For each time point one representative experiment of a minimum of 2 experiments is shown.