VCAM-1 expression in the BM during the course of G-CSF administration.

Mice were injected with either saline for 6 days (A) or G-CSF for 2 days (B), 4 days (C), or 6 days (D). In panels E and F, BM was taken 4 days after cessation of a 6-day course of G-CSF injection. Marrows were stained with MK/2-7 (A-E) or with an isotypic control (F). The red color shows VCAM-1 staining (× 250 magnification). Spleen weights from G-CSF– (●) and saline- (■) treated mice and the number of circulating CFCs and white blood cells (WBC) in the PB are shown in panels G, H, and I. Data represent mean ± SEM (6 mice/time point).