Recognition of the 4 peptides derived from pp65 by each CMV-specific CD8⁺ T-cell clone.

The results of ELISPOT assay with CMV-specific CD8⁺ T-cell clone no. 1 (A) and no. 3 (B) are shown. A total of 100 CD8⁺ T cells were cocultured with 50 000 T2-A24 cells in each well in the presence of each peptide at the indicated concentrations. The 2 peptides, no. 81 (●) and 83 (▪), that were recognized at picomolar levels share the amino acid sequence QYDPVAALF, with an additional C-terminal phenylalanine for no. 83. The amino acid sequences of peptides no. 80 (♦) and 82 (▴) are VYALPLKML and QYVKVYLESF, respectively.