Recognition by the QYDPVAALF-specific CD8⁺ T-cell clone of A24⁺ fibroblast cells infected either with CMV or recombinant vaccinia virus expressing pp65.

HLA-A24⁺ autologous and allogeneic fibroblast cells were mock- or CMV-infected and tested for stimulation of QYDPVAALF-specific CD8⁺ T-cell clone no. 1 in the ELISPOT assay. Autologous fibroblast cells infected with recombinant vaccinia virus expressing pp65 or glycoprotein B (gB) of CMV were also tested for stimulation of the T cells. A total of 200 (▪), 100 (), 50 (■), or 25 (▨) CD8⁺ T cells were cocultured with 10 000 fibroblast cells in each well. Each bar represents the average number of spots in duplicate wells.