HLA-A*2402–QYDPVAALF tetramers specifically bound to the relevant CD8⁺ T cells.

An HLA-A*2402–restricted EBV-specific CD8⁺ CTL clone (A), the HLA-A*2402–restricted QYDPVAALF-specific CD8⁺ CTL clone no. 1 (B), peripheral lymphocytes from a CMV-seronegative and HLA-A*2402⁺ donor (C), and those from the donor 5 in Figure 7 (D) were stained phycoerythrin-conjugated HLA-A*2402–QYDPVAALF tetrameric complex and an antibody to CD8 conjugated to Tricolor. The cells were analyzed by flow cytometry. The frequency of QYDPVAALF-specific CD8⁺ T cells in peripheral lymphocytes is shown as the percentage of the total CD8⁺ T cells (C,D).