Fig. 1.
Influence of DT388–GM-CSF and GM-CSF on ceramide metabolism in HL-60/VCR cells.
Cells, seeded in 6-well plates, were exposed to the agents at the indicated doses for 48 hours in medium containing [3H]palmitic acid. Radiolabeled ceramide was resolved from the total cell lipid extract by TLC and quantitated by liquid scintillation counting.