Fig. 9.
![Fig. 9. Influence of sphingomyelinase treatment on ceramide metabolism and protein synthesis. / (A,B) HL-60/VCR cells, prelabeled with [3H]palmitic acid, were incubated with sphingomyelinase for 4 hours, and lipids were extracted and analyzed. (C) To measure protein synthesis, cells were incubated with sphingomyelinase for 1 hour, before addition of [3H]leucine (1.0 μCI/well) for 3 hours. Data points represent the mean ± SD (n = 3). SD bars are not shown (error < 5%). SM indicates sphingomyelin.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/98/6/10.1182_blood.v98.6.1927/7/h81811534009.jpeg?Expires=1735813532&Signature=G8pjbux~vxAiFA38NQjDpFr4lt~cB0iKYfULzTYXSOALd6n9r~6ivnzj2WEn6L3CN4y4xRINl02hQwiDkcH3MLMy6Gh1eLXFib9a6JuNyAwsvqJhY07~I7PEnLYKriJdTNS2hgGlSj91fXqBAb0watLo31vvKMTo-3JvNjw0R~H3IxcogS~uDkqBxZhEvxD43x12c7WpvjaxClmz043wOofkDho1vPgpUvwRBELUUyLsP7JgVpuL01qTRjfKcWt7mjDJus2ba7M3--fhgIwWmQiMijsOuhPkiIX9rBVlcYXyBW3ObUVzE-gPBXf9C~qMu-9FM8AOx-RRe7sgxVk52Q__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Influence of sphingomyelinase treatment on ceramide metabolism and protein synthesis.
(A,B) HL-60/VCR cells, prelabeled with [3H]palmitic acid, were incubated with sphingomyelinase for 4 hours, and lipids were extracted and analyzed. (C) To measure protein synthesis, cells were incubated with sphingomyelinase for 1 hour, before addition of [3H]leucine (1.0 μCI/well) for 3 hours. Data points represent the mean ± SD (n = 3). SD bars are not shown (error < 5%). SM indicates sphingomyelin.
