In vivo recombination activity of the RAG1 mutants.

Harvested plasmids were analyzed for coding joint (CJ) formation by PCR. The PCR products were run on a 5% polyacrylamide gel and autoradiographed. Full-length wild-type (wt) and mutant alleles (R559S, R897X) were transfected into 293T cells with pEBG and deletion substrate pJ200. Lane 1: negative control; Lane 2: negative control; Lane 3: positive control (wild-type RAG1 cotransfected with wild-type RAG2); Lane 4: mutant R559S cotransfected with wild-type RAG2; Lane 5: mutant R897X cotransfected with wild-type RAG2.