Fig. 2.
Fig. 2. Expression of the klf12, klf4, and klfd genes in the zebrafish embryo. / Whole-mount in situ hybridization of zebrafish embryos with riboprobes for the zebrafish klf12 gene (A-C), klf4 (D-G), and klfd (H-J). Embryos are shown in lateral view, oriented with anterior to the left and dorsal up, except in panel D, where embryos are viewed obliquely from the posterior, with anterior to the top and dorsal to the right. (A-C) Expression of klf12. (A) Comparison of wild-type (wt, upper) and cloche mutant animals (clo, lower) at 24 hours past fertilization (hpf), showing the absence of klf12 expression in the intermediate cell mass (ICM, arrowheads) of clo embryos and klf12expression in hatching gland cells across the yolk (arrow). (B) Expression at 36 hpf showing hatching gland (arrow) and pronephric duct cell clusters (arrowhead). (C) Higher magnification and oblique view of animal in panel B, showing bilateral nature of pronephric duct cell clusters (arrowheads). (D-G) Expression of klf4. (D) Comparison of wt (left) and clo mutant (right) at 12 hpf in an oblique view, showing the absence of a row ofklf4-expressing blood cells in the posterior lateral plate mesoderm (arrowheads) and the position of the polster (arrow). (E) Comparison of wt (lower) and clo mutant animals (upper) at 24 hpf, showing the absence of klf4 expression in the ICM (arrowheads) of clo embryos and klf4 expression in hatching gland cells (arrow). Small arrowheads mark the primordia of the anterior and posterior lateral line ganglia. (F) At 48 hpf, circulating erythrocytes expressing klf4 are visible across the anterior of the yolk and in the vessels of the tail (arrowhead). The anterior and posterior extents of the migrating lateral line primordia are marked with small arrowheads. (G) Larva at 4 days past fertilization (dpf) showing klf4 expression in complete lateral line system. (H-J) Expression of klfd. (H) Comparison of wt (upper) and clo mutant animals (lower) at 24 hpf, showing the absence of klfd expression in the ICM (arrowheads) of clo embryos. (I) Embryo at 48 hpf showingklfd expression within circulating erythrocytes on anterior yolk and in trunk and tail vessels (arrowheads). (J) Larva at 8 dpf showing klfd expression in circulating definitive erythrocytes in tail vessels (arrowhead), heart lumen (small arrowhead), and pronephros (arrow). Original magnification 50 ×; stained with nitroblue tetrazolium chloride (NBT) and S-bromo-4-chloro-3-indolylphosphate p-toluidine salt (BCIP) precipitate.

Expression of the klf12, klf4, and klfd genes in the zebrafish embryo.

Whole-mount in situ hybridization of zebrafish embryos with riboprobes for the zebrafish klf12 gene (A-C), klf4 (D-G), and klfd (H-J). Embryos are shown in lateral view, oriented with anterior to the left and dorsal up, except in panel D, where embryos are viewed obliquely from the posterior, with anterior to the top and dorsal to the right. (A-C) Expression of klf12. (A) Comparison of wild-type (wt, upper) and cloche mutant animals (clo, lower) at 24 hours past fertilization (hpf), showing the absence of klf12 expression in the intermediate cell mass (ICM, arrowheads) of clo embryos and klf12expression in hatching gland cells across the yolk (arrow). (B) Expression at 36 hpf showing hatching gland (arrow) and pronephric duct cell clusters (arrowhead). (C) Higher magnification and oblique view of animal in panel B, showing bilateral nature of pronephric duct cell clusters (arrowheads). (D-G) Expression of klf4. (D) Comparison of wt (left) and clo mutant (right) at 12 hpf in an oblique view, showing the absence of a row ofklf4-expressing blood cells in the posterior lateral plate mesoderm (arrowheads) and the position of the polster (arrow). (E) Comparison of wt (lower) and clo mutant animals (upper) at 24 hpf, showing the absence of klf4 expression in the ICM (arrowheads) of clo embryos and klf4 expression in hatching gland cells (arrow). Small arrowheads mark the primordia of the anterior and posterior lateral line ganglia. (F) At 48 hpf, circulating erythrocytes expressing klf4 are visible across the anterior of the yolk and in the vessels of the tail (arrowhead). The anterior and posterior extents of the migrating lateral line primordia are marked with small arrowheads. (G) Larva at 4 days past fertilization (dpf) showing klf4 expression in complete lateral line system. (H-J) Expression of klfd. (H) Comparison of wt (upper) and clo mutant animals (lower) at 24 hpf, showing the absence of klfd expression in the ICM (arrowheads) of clo embryos. (I) Embryo at 48 hpf showingklfd expression within circulating erythrocytes on anterior yolk and in trunk and tail vessels (arrowheads). (J) Larva at 8 dpf showing klfd expression in circulating definitive erythrocytes in tail vessels (arrowhead), heart lumen (small arrowhead), and pronephros (arrow). Original magnification 50 ×; stained with nitroblue tetrazolium chloride (NBT) and S-bromo-4-chloro-3-indolylphosphate p-toluidine salt (BCIP) precipitate.

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