Expression of klf2bin the pharyngula and hatching stages of the zebrafish embryo.

Whole-mount in situ hybridization of zebrafish embryos with riboprobe for the zebrafish klf2b gene. Embryo in panel A is viewed laterally with anterior to the left and dorsal up; embryos in panels B-C are viewed from the dorsal aspect with anterior up. Scale bars are 100 μm (A) and 50 μm (B-C). (A) Embryo at 24 hours past fertilization (hpf) showing klf2b expression in squamous cells of the epidermis. (B) Embryo at 36 hpf at the axial level of the anterior trunk (somites are visible as dark blocks) showing bilateralklf2b-expressing cords of cells extending laterally from anterior margin of the pectoral fin bud (arrowheads). (C) Embryo at 48 hpf viewed as in panel B, showing expression of klf2b in center of pectoral fin bud. Original magnification in panel A 100 ×, panels B and C 200 ×; stained with NBT/BCIP precipitate.