Detection of EBER-1⁺ cells in lymphocyte subpopulations in patients with EBV-HLH.

PBMNCs obtained from patient EBV-HLH2 at 8 days after onset were separated into CD4⁺ T cells (A), CD8⁺ T cells (B), CD16⁺ NK cells (C), or CD20⁺ B cells (D) by electronic sorting using EPICS-Elite as described. The presence of EBER-1 mRNA was detected by ISH in each cytocentrifuged cell subpopulation. The arrows indicate EBER-1⁺cells. Original magnification × 400.