Lymphocyte phenotype of EBV-HLH and CAEBV.

Peripheral lymphocytes from EBV-HLH and CAEBV patients were stained with PE-conjugated anti-CD4, CD8, MoAbs in combination with FITC-conjugated anti-CD45RO or HLA-DR MoAbs and analyzed with flow cytometry. PE-conjugated anti-CD16 MoAb was used only with FITC-conjugated anti–HLA-DR MoAb. ▪ indicates the percentages of HLA-DR⁺ andindicates the percentages of CD45RO⁺ cells in CD4⁺ T cells, CD8⁺ T cells, and CD16⁺ NK cells. Increase of activated cells was noted in subpopulations in which EBV infection was detected.