LPS induction of TF and TNF-α expression in PBMCs is inhibited by the MEK inhibitor PD98059.

PBMCs were preincubated by various doses of PD98059 (5, 10, 20 μM) for 30 minutes prior to stimulation with LPS (100 ng/mL) for 5 hours. (A) TF activity was determined in triplicate using a one-step clotting assay. (B) TNF-α antigen in cell supernatants was determined in duplicate by ELISA. These data represents the mean ± SD from 3 independent experiments.