Fig. 5.
Fig. 5. Regulation of TF and TNF-α by Egr-1. / (A) Total RNA was isolated from THP-1 cells stimulated with LPS (0-24 hours). Levels of Egr-1, TF, and G3PDH mRNA were determined by Northern blotting. (B) THP-1 cells were transfected with plasmids containing either the wild-type TF promoter (wt) or an Egr-1 mutant (Egr-1M) as well as the wild-type TNF-α promoter (wt) or an Egr-1M. Transfected cells were incubated with or without LPS for 5 hours at 37°C. Luciferase activity in cell lysates was determined, and the results were expressed as fold induction. Data (mean ± SD) are from at least 3 independent experiments.

Regulation of TF and TNF-α by Egr-1.

(A) Total RNA was isolated from THP-1 cells stimulated with LPS (0-24 hours). Levels of Egr-1, TF, and G3PDH mRNA were determined by Northern blotting. (B) THP-1 cells were transfected with plasmids containing either the wild-type TF promoter (wt) or an Egr-1 mutant (Egr-1M) as well as the wild-type TNF-α promoter (wt) or an Egr-1M. Transfected cells were incubated with or without LPS for 5 hours at 37°C. Luciferase activity in cell lysates was determined, and the results were expressed as fold induction. Data (mean ± SD) are from at least 3 independent experiments.

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