Morphology and ultrastructure of CD86⁻ BM DCs and CD86⁺ BM DCs.

CD86⁻ DCs exhibited short, blunt prolongations (A), a round nucleus with prominent nucleoli, multiple cytoplasmic vesicles, mitochondria, few lysosomes (C), and typical “veils” (E). After maturation, CD86⁺ DCs showed a typical dendritic morphology, with an eccentric, indented nucleus (B, D) and a veiled surface with delicate filamentous projections with knoblike tips (F, arrow). (A-B) May-Grünwald-Giemsa. (C-D) TEM ×6000. (E-F) SEM ×3500). Bar, 5 μm.