Th-cell–driving potential of BM DCs terminally differentiated by LPS or CD40 ligation.

Detection of Th1 (IFN-γ) and Th2 cytokines (IL-4 and IL-10) in C3H CD3⁺CD4⁺ splenic T cells after in vitro stimulation with γ-irradiated allogeneic (B10): (1) CD86⁺BM DCs (A-D); (2) CD86⁺ BM DCs differentiated with anti-CD40 IgM mAbs (E-H); or (3) CD86⁺ BM DCs incubated with LPS (I-L). After 3-day MLR, the T cells were harvested and restimulated with anti-CD3ε + anti-CD28 mAbs in the presence of brefeldin A. Thereafter, the cells were labeled with anti-CD3, FITC anti-CD4, and PE anti–IFN-γ, anti–IL-4, or anti–IL-10 mAbs. DCs were gated out according to their lack of expression of CD3. As controls, C3H T cells were maintained for 3 to 4 days in low-dose rIL-2 (2 U/mL) and then restimulated with anti-CD3 + anti-CD28 mAbs (M-P). Irrel indicates irrelevant. Figures in quadrants denote percentages. Data are representative of 3 separate experiments.