Effects of alanine-substituted FANCCmutations on STAT1 phosphorylation in PD4 cells.

Upper panel: PD4 alanine-substituted FANCC mutant cells (lanes 5-16) were exposed to 1 ng/mL IFN-γ for times indicated and compared to PD4 cells (lanes 1 and 2) as well as PD4/FANCC cells complemented by transduction with wild-type FANCC (lanes 3 and 4). Cell lysates were separated by SDS-PAGE in a 7.5% polyacrylamide gel and immunoblotted with an antibody specific for tyrosine phosphorylated STAT1 protein. Lower panel: The blot described in the upper panel was stripped and reprobed for total STAT1 protein.