Fig. 8.
Effect of alanine-substituted FANCCmutations on IFN-γ–induced formation of STAT1–IFN-γRα complex in B lymphocytes.
Cells were stimulated with IFN-γ for 10 minutes and solubilized in digitonin lysis buffer as described in “Materials and methods.” The WCLs were then incubated with anti–IFN-γRα antibody. The immunoprecipitates were analyzed by SDS-PAGE followed by immunoblotting. Upper panel: Cell lysates prepared from HSC536N isogenic lines were immunoprecipitated with anti–IFN-γRα and probed with anti-STAT1 monoclonal antibody. Lower panel: The blot described in the top panel was stripped and reprobed with anti–IFN-γRα.
