Alanine mutants S249A and E251A sensitize hematopoietic cells to IFN-γ and TNF-α cytotoxicity.

HSC536N lymphoblasts expressing normal FANCC or mutatedFANCC were incubated with IFN-γ (10 ng/mL) for 15 hours before treatment with 10 ng/mL TNF-α for 48 hours and harvested for flow cytometric analysis (▪, treated; □, untreated). Treatments of HSC/VEC and HSC/FANCC with Camptothecin were used as positive controls, 50 mM for 9 hours) and induced positivity of 65.1% and 22.5%, respectively. Total events of 10 000 for each sample were analyzed.