Effect of anti-CD4 and anti-CD8 on cytotoxic activities of mice spleen cells immunized with HSPs against A20.
After BMT, 40 μg HSP70 (A) or 5 μg GP96 (B) derived from A20 was injected subcutaneously on days 18 and 25. MLTC was examined on day 32 with spleen cells from mice in these groups, and the specific cytotoxicity against A20 cells was determined by 51Cr release assay on day 37. Mice spleen cells were pretreated with anti-CD4 or -CD8 before cytotoxic assay. Spleen cells derived from HSP70- or GP96-immunized mice showed significant cytotoxicities against A20. These cytotoxicities were significantly blocked by anti-CD8 but not by anti-CD4. Each point represents the mean of triplicate samples. Data are representative of 3 separate experiments. **P < .01 versus PBS. *P < .01 versus HSP70 derived from A20. †P < .01 versus GP96 derived from A20;t test.
