Fig. 6.
Effect of anti–MHC class I on cytotoxic activities of mice spleen cells immunized with HSPs against A20.
After BMT, 40 μg HSP70 (A) or 5 μg GP96 (B) derived from A20 was injected subcutaneously on days 18 and 25, and the specific cytotoxicity against A20 cells was determined by 51Cr release assay on day 37 as described in “Materials and methods.” A20 cells were pretreated with anti–MHC class I before cytotoxic assay. Cytotoxicities of the spleen cells derived from HSP70- or GP96-immunized mice against A20 were significantly blocked by anti–MHC class I. Each point represents the mean of triplicate samples. *P < .01 versus HSP70 derived from A20. †P < .01 versus GP96 derived from A20,t test.
