Proliferative responses of β₂GPI-specific CD4⁺ T cell clones to various β₂GPI preparations.

T-cell clones were cultured with autologous APCs for 3 days in medium alone or in medium supplemented with GP-F, GP1, GP2, GP3, native β₂GPI, reduced β₂GPI, MalBP (10 μg/mL), TT (5 μg/mL), or PHA (1 μg/mL), and antigen-induced T-cell proliferation was measured by ³H-thymidine incorporation. Significant T-cell proliferation to recombinant β₂GPI or reduced β₂GPI in comparison with the respective controls is shown as an asterisk. A representative result of 3 independent experiments is shown.